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SUMMARY:Building a Spatial and Transcriptomic Framework for Transcription 
 Factor Regulation in Wolffia australiana
DTSTART;VALUE=DATE-TIME:20260929T130000Z
DTEND;VALUE=DATE-TIME:20260929T131500Z
DTSTAMP;VALUE=DATE-TIME:20260915T141156Z
UID:indico-contribution-2905@cern.ch
DESCRIPTION:Speakers: Kevin Cox (Washington University in St. Louis/Danfor
 th Center)\nUnderstanding how plants grow\, develop\, and respond to their
  environment requires not only identifying the genes involved\, but functi
 onally characterizing what each gene does. To understand how transcription
  factors regulate genes across tissues and organs\, transcriptional data n
 eeds to be placed within a spatial\, cellular\, and organismal context. *W
 olffia australiana* is an attractive system for addressing this fundamenta
 l question due to its small body plan\, fast clonal growth rates\, and com
 pact non-redundant genome. In our lab\, we use high-resolution\, 3D imagin
 g technologies and transcriptomics to pinpoint the localization of transcr
 iption factors and how they globally regulate genes in a whole plant. We u
 sed X-ray microscopy to capture the 3D architecture of *W. australiana*\, 
 resolving the structure of the meristematic region and laying the groundwo
 rk for future cell segmentation and quantification. We further employed ex
 pansion microscopy\, which enables nanoscale imaging beyond the diffractio
 n limit\, achieving roughly 4x physical expansion of intact *W. australian
 a* and enhanced resolution of nuclei and chloroplasts. Together\, these im
 aging approaches provide a spatial and cellular scaffold for mapping trans
 criptional activity across the whole plant. To begin applying this framewo
 rk\, we examined the transcriptomic response to early salt stress by gener
 ating a bulk RNA-sequencing atlas over a 48-hour timecourse\, which identi
 fied specific transcription factors and functional pathways underlying the
  salt stress response. These imaging and transcriptomic approaches togethe
 r lay the groundwork for localizing transcription factor activity within a
  defined spatial and cellular context in *W. australiana*.\n\nhttps://indi
 co.unina.it/event/117/contributions/2905/
LOCATION:Department of Agricultural Sciences of the University of Napoli F
 ederico II\, Portici\, Italy Sala Cinese
URL:https://indico.unina.it/event/117/contributions/2905/
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