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SUMMARY:Potential cobalamin-producing Wolffia globosa-associated microbiom
 e
DTSTART;VALUE=DATE-TIME:20260930T073000Z
DTEND;VALUE=DATE-TIME:20260930T074500Z
DTSTAMP;VALUE=DATE-TIME:20260920T030933Z
UID:indico-contribution-2912@cern.ch
DESCRIPTION:Speakers: Arinthip Thamchaipenet (Kasetsrat University)\nPoten
 tial cobalamin (vitamin B12)-producing *Wolffia globosa*-associated bacter
 ia were investigated through shotgun metagenomic sequencing analysis. Taxo
 nomic classification assigned microbiomes consisting of 5 phyla\, 8 classe
 s\, 36 families\, and 68 genera. High-quality metagenome-assembled genomes
  (MAGs) were assigned to *Allorhizobium* sp.\, *Alsobacter* sp.\, *Bosea* 
 sp.\, *Bradyrhizobium* *huaxiensis*\, *Brevundimonas* sp.\, *Methylophilus
 * sp.\, *Microbacterium* sp.\, *Mycobacterium* sp.\, *Neoroseomonas* *mari
 na*\, *Nevskia* sp.\, *Novosphingobium* sp.\, *Phenylobacterium* sp.\, *Ps
 eudonocardia* *carboxydivorans*\, *Rhizobium* sp.\, *Rhizorhabdus* sp.\, *
 Roseomonas* sp.\, *Sphingomonas* sp.\, and *Xanthomonas* *sontii*. Functio
 nal annotation across all MAGs revealed potential pathways related to arom
 atic compound degradation\, cofactor and vitamin metabolism\, serine and t
 hreonine metabolism\, nitrogen metabolism\, and other carbohydrate metabol
 ic processes. Several MAGs contained complete modules of cobalamin biosynt
 hesis including *hem*\, *cob*\, *cbi* genes responsible for corrin ring sy
 nthesis\, cobalt transport\, lower-ligand formation\, and final cobalamin 
 assembly. Interestingly\, the definitive molecular marker for active B12 p
 roduction\, *bluB*\, was identified in several MAGs. Subsequently\, eight 
 bacterial genera were isolated using culture dependent methods and three n
 ovel species have been proposed. Co-cultivation experiments are underway t
 o understand the associated B12 production of *W. globosa* holobionts.\n\n
 https://indico.unina.it/event/117/contributions/2912/
LOCATION:Department of Agricultural Sciences of the University of Napoli F
 ederico II\, Portici\, Italy Sala Cinese
URL:https://indico.unina.it/event/117/contributions/2912/
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