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SUMMARY:Self-DNA Effects on Growth\, Oxidative Stress\, and Nutritional Qu
 ality of Lemna perpusilla: In Vitro
DTSTART;VALUE=DATE-TIME:20261002T080500Z
DTEND;VALUE=DATE-TIME:20261002T082500Z
DTSTAMP;VALUE=DATE-TIME:20260915T180330Z
UID:indico-contribution-2940@cern.ch
DESCRIPTION:Speakers: Jesis Silvano (IPB University)\nDuckweed (Lemna sp.)
  is a promising alternative ruminant feed due to its rapid growth and high
  protein content. However\, extracellular self-DNA released from plant tis
 sues may exert species-specific inhibitory effects on microbial activity\,
  a phenomenon remaining unexplored in rumen systems. This study evaluated 
 the effects of Lemna supplementation and Lemna-derived self-DNA on in vitr
 o rumen fermentation characteristics. Five dietary treatments were evaluat
 ed: P0 (basal diet: 60% forage\, 40% concentrate)\; P1 (60% forage + 36% c
 oncentrate + 4% Lemna self-DNA)\; P2 (60% forage + 36% concentrate + 4% Le
 mna control)\; P3 (50% forage + 30% concentrate + 20% Lemna self-DNA)\; an
 d P4 (50% forage + 30% concentrate + 20% Lemna control). Rumen pH\, $\\tex
 t{NH}_3\\text{-N}$\, total volatile fatty acids (VFA)\, and VFA profiles w
 ere determined post-in vitro incubation. Rumen pH and $\\text{NH}_3\\text{
 -N}$ concentrations were not significantly affected by treatments (P > 0.0
 5). Conversely\, total VFA production and composition were significantly a
 ltered (P < 0.05). The highest total VFA concentration was observed in P3\
 , followed by P2 and P1\, indicating enhanced fermentation with higher Lem
 na inclusion. Notably\, treatments containing self-DNA tended to produce l
 ower fermentation responses than their respective controls\, suggesting a 
 potential inhibitory effect on microbial activity. Additionally\, acetate 
 proportion decreased while propionate and butyrate increased\, shifting to
 ward more efficient fermentation pathways. In conclusion\, while Lemna nut
 rients stimulate rumen fermentation\, its self-DNA partially modulates mic
 robial activity through inhibitory mechanisms\, highlighting the importanc
 e of considering both nutritional and molecular factors in plant biomass e
 valuation.\n\nhttps://indico.unina.it/event/117/contributions/2940/
LOCATION: Sala Monumentini
URL:https://indico.unina.it/event/117/contributions/2940/
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