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SUMMARY:Species-Specific Growth Inhibition by Extracellular Self-DNA in a 
 Two-Species Duckweed Community and Its Association with Oxidative Stress
DTSTART;VALUE=DATE-TIME:20260929T155500Z
DTEND;VALUE=DATE-TIME:20260929T161500Z
DTSTAMP;VALUE=DATE-TIME:20260916T130420Z
UID:indico-contribution-2941@cern.ch
DESCRIPTION:Speakers: Wilhelmus Terang Arga Sanjaya (7Department of Soil S
 cience and Land Resources\, Faculty of Agriculture\, IPB University)\nExtr
 acellular self-DNA has emerged as a species-specific regulator of plant gr
 owth\, yet its effects and physiological responses in aquatic plants remai
 n poorly understood. This study investigated whether extracellular self-DN
 A preferentially inhibits growth in a two-species duckweed community and w
 hether this response is associated with oxidative stress. Lemna perpusilla
  and Spirodela polyrhiza were exposed to self-DNA\, non-self DNA\, and mix
 ed DNA. A dose–response assay using genomic DNA solutions ranging from 0
  to 2\,000 ng µL⁻¹ identified the most inhibitory treatment. Growth wa
 s evaluated using frond number\, root number\, root length\, fresh biomass
 \, and dry biomass. Species specificity was examined by co-cultivating bot
 h species and exposing the culture separately to DNA from each species. Ph
 ysiological responses to self-DNA at 1\,000 ng µL⁻¹ were assessed thro
 ugh reactive oxygen species (ROS)\, superoxide dismutase (SOD)\, peroxidas
 e (POD)\, catalase (CAT)\, and malondialdehyde (MDA). Self-DNA caused stro
 nger inhibition than non-self DNA and mixed DNA. In the two-species commun
 ity\, L. perpusilla DNA reduced L. perpusilla fresh biomass by 76.5% but S
 . polyrhiza by only 3.2%. Conversely\, S. polyrhiza DNA reduced S. polyrhi
 za fresh biomass by 93.3% but L. perpusilla by only 14.5%\, demonstrating 
 reciprocal species-preferential inhibition. Self-DNA also increased ROS ac
 cumulation by 39% in L. perpusilla and 54% in S. polyrhiza\, accompanied b
 y enhanced SOD\, POD\, and CAT activities and elevated MDA levels. These f
 indings show that extracellular self-DNA preferentially inhibits its sourc
 e species within a mixed duckweed community and that this response is asso
 ciated with oxidative stress\, supporting investigation of self-DNA recogn
 ition and species-targeted aquatic plant management.\n\nhttps://indico.uni
 na.it/event/117/contributions/2941/
LOCATION: Sala Monumentini
URL:https://indico.unina.it/event/117/contributions/2941/
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