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SUMMARY:Small RNA biogenesis in Duckweeds
DTSTART;VALUE=DATE-TIME:20260929T155500Z
DTEND;VALUE=DATE-TIME:20260929T161500Z
DTSTAMP;VALUE=DATE-TIME:20260917T105241Z
UID:indico-contribution-2946@cern.ch
DESCRIPTION:Speakers: Filipp Krasnovid (Gregor Mendel institute\, Vienna B
 iocenter PhD Programm)\nSmall RNA silencing pathways in plants play crucia
 l roles in development\, antiviral resistance\, and transposon control. Th
 ere are two types of silencing: transcriptional gene silencing (TGS) and p
 ost-transcriptional (PTGS). TGS acts via the RNA-directed DNA methylation 
 pathway and 24nt-long small interfering RNAs (siRNAs). PTGS functions at t
 he mRNA level\, where viral or transgene RNA transcripts trigger the forma
 tion of 21nt- and 22nt-long siRNAs that target AGO proteins toward these t
 ranscripts leading to their cleavage and degradation.\n\nDuckweeds have si
 mplified their silencing pathways and challenge the current model of PTGS 
 in several ways. Dicer-like protein 2 (DCL2)\, the protein responsible for
  the production of 22nt siRNA\, conserved among plants\, is lost in duckwe
 eds\, yet we observe 22nt siRNA in all three species studied in our lab. T
 ransgenic silencing in Lemna minor is characterised by the equal presence 
 of both 21nt- and 22nt-long siRNAs\; transient expression of an inverted r
 epeat (IR) in *Spirodela polyrhiza*  and in *Wolffia brasiliensis* results
  in the production of both 21nt and 22nt siRNA derived from this IR. These
  22nt siRNAs\, in turn\, do not seem to trigger transitivity\, the silenci
 ng amplification process\, despite the presence of all the necessary eleme
 nts that we know of. \n\nThe goal of our project is to understand the biog
 enesis of 22nt-long siRNA and find the mechanisms responsible for the diff
 erence in PTGS functioning in duckweeds\, including transitivity — a phe
 nomenon that is not well understood even in conventional models such as Ar
 abidopsis.\n\nhttps://indico.unina.it/event/117/contributions/2946/
LOCATION: Sala Monumentini
URL:https://indico.unina.it/event/117/contributions/2946/
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