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SUMMARY:Cellular-level visualization of salinity stress responses in Wolff
 ia australiana
DTSTART;VALUE=DATE-TIME:20260929T155500Z
DTEND;VALUE=DATE-TIME:20260929T161500Z
DTSTAMP;VALUE=DATE-TIME:20260916T130421Z
UID:indico-contribution-2947@cern.ch
DESCRIPTION:Speakers: Hayley Sussman ()\n*Wolffia australiana* offers a un
 ique opportunity to study spatial variation in stress responses given a sm
 all portion of its tissue is exposed to air while the remainder is submerg
 ed in water\, creating distinct above- and below-water regions within a ph
 ysically condensed space (<1 mm in diameter). As a result\, in a high sali
 ne environment\, the below-water region has direct exposure to sodium chlo
 ride\, while the above-water tissue does not. Previous research has shown 
 which gene regulatory networks are induced during salinity stress across t
 he whole tissue of a variety of duckweed species. However\, the spatial or
 ganization of these transcriptomic responses has not been elucidated. As a
  first step towards addressing this question\, we sought to identify where
  sodium ions accumulate spatially and the corresponding physiological impa
 cts.   To visualize the cellular-level physiological impact of salinity st
 ress on *W. australiana*\, this project uses fluorescent stains and indica
 tors under confocal microscopy. CoroNa Green\, a cell-permeant compound th
 at fluoresces upon binding sodium ions\, is used to spatially observe sodi
 um ion accumulation. In tandem\, we spatially observe and quantify both ch
 lorophyll autofluorescence and reactive oxygen species production as proxi
 es for photosynthetic productivity and oxidative stress\, respectively. To
 gether\, these measurements reveal cellular-level differences in stress ex
 posure\, suggesting compartmentalized stress responses between the above- 
 and below-water tissues.\n\nhttps://indico.unina.it/event/117/contribution
 s/2947/
LOCATION: Sala Monumentini
URL:https://indico.unina.it/event/117/contributions/2947/
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