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SUMMARY:Dietary supplementation with Lemna minor mitigates the metabolic a
 lterations induced by ingestion of PLA nanoplastic in a rat model
DTSTART;VALUE=DATE-TIME:20260929T155500Z
DTEND;VALUE=DATE-TIME:20260929T161500Z
DTSTAMP;VALUE=DATE-TIME:20260916T130451Z
UID:indico-contribution-2955@cern.ch
DESCRIPTION:Speakers: Martina Lombardi (University of Salerno\, Dept. Medi
 cine\, Surgery and Dentistry\; "Scuola Medica Salernitana")\, Andrea Viggi
 ano (University of Salerno\, Dept. Medicine\, Surgery and Dentistry\; "Scu
 ola Medica Salernitana")\nPolylactic acid (PLA) has gained considerable in
 dustrial relevance\, often considered an environmentally preferable materi
 al\, but it can actually undergo fragmentation\, generating micro- and nan
 o-plastics (MPs/NPs)\, whose biological consequences are still insufficien
 tly characterized. In the present study\, we used an untargeted metabolomi
 cs approach to investigate the in vivo metabolic alterations associated wi
 th chronic oral exposure to PLA-NPs and to determine whether supplementati
 on with Lemna minor (LM) could modulate the metabolic response to PLA-NPs.
  Wistar rats were randomly allocated to four experimental groups: untreate
 d controls (CTR)\, oral PLA-NPs exposure (0.05 mg/ml in drinking water)\, 
 dietary LM supplementation (20g/day)\, and combined PLA-NP and LM treatmen
 t. Fecal samples were collected at baseline (T0) and after 14 (T1) and 21 
 days (T2) of treatment and analyzed by liquid chromatography–mass spectr
 ometry (LC–MS) using a high-resolution Orbitrap mass spectrometer. PLA-N
 Ps exposure induced a broad metabolic rearrangement\, mainly involving pat
 hways related to bile acid homeostasis\, energy regulation and microbial m
 etabolism. Co-supplementation with LM partially counteracted the metabolic
  alterations induced by PLA-NPs. At T1\, LM shifted bile acids\, branched-
 chain fatty acids\, and acylcarnitines toward CTR levels\, suggesting modu
 lation of bile acid homeostasis\, microbial fermentation\, and lipid utili
 zation. At T2\, partial normalization extended to metabolites involved in 
 carbohydrate\, lipid\, and nucleotide metabolism. Although residual differ
 ences from untreated CTR persisted\, these data support a partial rescue o
 f the PLA-NP-associated metabolic phenotype. Collectively\, our findings d
 emonstrate that PLA-NPs can induce profound disturbances in metabolic home
 ostasis and provide a strong rationale for further investigating LM as a p
 otential nutritional countermeasure.\n\nhttps://indico.unina.it/event/117/
 contributions/2955/
LOCATION: Sala Monumentini
URL:https://indico.unina.it/event/117/contributions/2955/
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