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SUMMARY:Haplotype-resolved genome assembly and single-nucleus sequencing p
 rovide insights into the simplest flower development in Wolffia microscopi
 ca
DTSTART;VALUE=DATE-TIME:20261002T080500Z
DTEND;VALUE=DATE-TIME:20261002T082500Z
DTSTAMP;VALUE=DATE-TIME:20260915T211946Z
UID:indico-contribution-2956@cern.ch
DESCRIPTION:Speakers: Abhisek Chakraborty (Johannes Gutenberg University o
 f Mainz\, Germany)\nEvolution does not always lead to increased complexity
 \, and in plants\, the loss of floral organs is a recurring phenomenon acr
 oss different lineages. However\, the mechanisms behind this simplificatio
 n remain poorly understood. To investigate the evolutionary processes lead
 ing to floral organ loss\, duckweeds\, especially *Wolffia microscopica*\,
  provide an ideal system because of the smallest flowers with the simplest
  morphology – with a single stamen and a unicarpellate gynoecium\, which
  are even simpler than the model species *Arabidopsis thaliana*\, yet with
  essential genetic makeups. Therefore\, by integrating single-nucleus mult
 i-omics with comparative genomics\, we ask the specific questions: i) whic
 h floral cell-types remain in this smallest flowering plant? and ii) which
  cell-types and genes are showing evolutionary signatures? \n\nWe construc
 ted a haplotype-resolved genome assembly of *W. microscopica* with a total
  size of 666.5 Mb\, consisting of 20 chromosomes and 12\,623 protein-codin
 g genes. We performed single-nucleus RNA-Seq and single-nucleus ATAC-Seq (
 10\,720 nuclei) to identify all cell-types\, including the male and female
  floral cell-types. We identified the marker genes and the transcription f
 actor-binding motifs enriched in the floral cell-types\, which could be re
 sponsible for establishing and maintaining the floral cell-type identities
 . Further\, we performed natural selection and gene family evolution analy
 ses to understand the evolutionary mechanisms underlying the development o
 f the world's simplest flower. The detailed single-nucleus multi-omics and
  evolutionary insights aid in uncovering the fundamental genetic mechanism
 s and regulatory networks responsible for the development of the simplest 
 flowers\, which likely represent the minimal controls of sexual reproducti
 on in plants.\n\nhttps://indico.unina.it/event/117/contributions/2956/
LOCATION: Sala Monumentini
URL:https://indico.unina.it/event/117/contributions/2956/
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