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SUMMARY:Investigating siRNA-mediated silencing in duckweeds\, a potential 
 model for translational repression in plants
DTSTART;VALUE=DATE-TIME:20261002T080500Z
DTEND;VALUE=DATE-TIME:20261002T082500Z
DTSTAMP;VALUE=DATE-TIME:20260916T020036Z
UID:indico-contribution-2961@cern.ch
DESCRIPTION:Speakers: Laura Diezma-Navas (Gregor Mendel Institute)\nIn euk
 aryotes\, post-transcriptional gene silencing (PTGS) involves the producti
 on of small\ninterfering RNAs (siRNAs) and their incorporation into ARGONA
 UTE (AGO) proteins. The\nresulting siRNA–AGO complex mediates mRNA cleav
 age and/or translational repression\,\nleading to degradation via RNA deca
 y pathways and ultimately resulting in reduced protein\nexpression. In pla
 nts\, mRNA cleavage is believed to be the main mode of action of siRNAAGO 
 complexes\, and although translational repression has been shown\, it is n
 ot yet well\nunderstood. Interestingly\, in duckweeds\, a group of small a
 quatic flowering plants\, siRNAmediated transgene silencing causes strong 
 loss of protein synthesis\, while mRNA levels\nremain largely unchanged. O
 n the contrary\, loss of siRNA results in resoration of protein\nlevels. T
 his observation suggests that translational repression\, rather than mRNA\
 ndegradation\, may be a predominant mechanism of gene regulation in this g
 roup of plants.\nTo investigate this hypothesis\, we are exploiting transg
 ene silencing in duckweeds to\nanalyze the fate and turnover of mRNAs\, ta
 rgeted or not by siRNAs\, using a combination of\npolysome profiling\, SLA
 M-seq\, and ribosome footprinting. Understanding silecing\nmechanisms in d
 uckweeds will expand the knowledge of silencing mechanisms\, mostly\ninves
 tigated in a handful of plant models\, and potentially provide further ins
 ights into their\ntranslational repression mechanisms.\n\nhttps://indico.u
 nina.it/event/117/contributions/2961/
LOCATION: Sala Monumentini
URL:https://indico.unina.it/event/117/contributions/2961/
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