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SUMMARY:Cobalt-Driven Restructuring of the Duckweed Endophytic Microbiome 
 Enhances Bioavailable Vitamin B₁₂
DTSTART;VALUE=DATE-TIME:20261002T080500Z
DTEND;VALUE=DATE-TIME:20261002T082500Z
DTSTAMP;VALUE=DATE-TIME:20260915T223808Z
UID:indico-contribution-2962@cern.ch
DESCRIPTION:Speakers: Dalia Rishmawi ()\nVitamin B₁₂ (cobalamin) is es
 sential for animal and human metabolism\, while plants neither synthesize 
 nor require it. Yet\, aquatic plants such as duckweeds can accumulate bioa
 vailable B₁₂ synthesized by their endophytic microbiome. We hypothesiz
 ed that cobalt\, the central metal ion in cobalamin would enhance B₁₂ 
 accumulation by altering endophytic community structure and function. To t
 est our hypothesis\, we cultivated the rooted duckweed species Lemna minor
  and Spirodela polyrhiza and the rootless species Wolffia globosa across a
  cobalt gradient\, ranging from 0 to 1 µM (just below the reported toxici
 ty threshold). We quantified growth and bioavailable B12\, profiled the co
 rresponding endophytic bacterial communities and examined B₁₂ partitio
 ning between roots and fronds.\nResponses to cobalt were species-specific.
  In L. minor\, B₁₂ increased by up to fivefold without affecting growt
 h rate. Cobalt explained 54% of the variation in bacterial community compo
 sition and significantly altered the abundance of Novosphingobium and Rose
 omonas. In S. polyrhiza\, B₁₂ increased by up to threefold\, while cob
 alt explaining 38% of the variation in community composition and significa
 ntly influencing alpha diversity and overall community structure. In W. gl
 obosa\, B₁₂ increased significantly at the highest cobalt concentratio
 n without detectable changes in community composition\, suggesting regulat
 ion through microbial activity rather than taxonomic replacement. B₁₂ 
 localization was also species-specific\, with significantly greater accumu
 lation in the roots of S. polyrhiza than in those of L. minor. These findi
 ngs demonstrate that cobalt enhances microbiome derived B₁₂ accumulati
 on in duckweed and provides a practical strategy for improving the nutriti
 onal value of duckweed biomass.\n\nhttps://indico.unina.it/event/117/contr
 ibutions/2962/
LOCATION: Sala Monumentini
URL:https://indico.unina.it/event/117/contributions/2962/
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