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SUMMARY:Influence of recipient gas-exchange capacity on axenic in vitro cu
 ltivation of Wolffia arrhiza
DTSTART;VALUE=DATE-TIME:20260929T160000Z
DTEND;VALUE=DATE-TIME:20260929T161500Z
DTSTAMP;VALUE=DATE-TIME:20261009T143254Z
UID:indico-contribution-2986@cern.ch
DESCRIPTION:Speakers: Zoé Faes (Department of Plants and Crops\, Ghent un
 iversity\, Belgium)\nThe rising demand for sustainable food sources has br
 ought attention to unconventional and innovative alternatives like duckwee
 d\, a small aquatic plant recognized for its rapid growth rate and high nu
 tritional value. To initiate large-scale cultivation\, sufficient sterile 
 biomass must first be produced from axenic in vitro stock cultures. Theref
 ore\, this study evaluated the effect of recipient gas exchange capacity o
 n relative growth rate (RGR) and nutrient medium pH for two W. arrhiza clo
 nes at 7 and 14 days of in vitro cultivation. Plants were grown axenically
  in liquid N-medium with an adjusted NO3-/NH4+ ratio of 90/10\, under cont
 rolled environmental conditions (25.2 ± 0.8 °C\, 150 µmol m⁻² s⁻¹
 \, 50% RH\, 16:8 h light:dark)\, with five biological replicates per treat
 ment. Four recipient types were used: sterile parafilm-sealed jars (no gas
  exchange) and Microbox Eco2 containers with increasing gas exchange capac
 ity (L\, XL\, XXL). One clone generally showed higher RGR than the other. 
 Recipient effects on RGR showed no uniform significant differences among g
 as-exchange treatments. Medium pH decreased over time in all treatments\, 
 consistent with preferential NH₄⁺ uptake and associated proton release
  into the medium. These results demonstrate that gas exchange influences i
 n vitro Wolffia cultivation\, mainly through changes in medium pH\, while 
 clone identity remains a dominant driver of growth performance\n\nhttps://
 indico.unina.it/event/117/contributions/2986/
LOCATION: Sala Monumentini
URL:https://indico.unina.it/event/117/contributions/2986/
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