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SUMMARY:Small meets smaller: rewiring small RNA silencing in duckweeds
DTSTART;VALUE=DATE-TIME:20260929T120000Z
DTEND;VALUE=DATE-TIME:20260929T121500Z
DTSTAMP;VALUE=DATE-TIME:20260916T061314Z
UID:indico-contribution-429-2973@cern.ch
DESCRIPTION:Speakers: Arturo Marí-Ordóñez (Gregor Mendel Institute)\nSm
 all RNA pathways play important regulatory and genome defense roles in pla
 nts\, 21-to-24-nt short interfering (si)RNAs and micro (mi)RNAs guide the 
 silencing of transposable elements (TEs)\, viruses and endogenous genes\, 
 acting both at the transcriptional gene silencing level (TGS)\, through RN
 A-directed DNA methylation (RdDM)\, and post-transcriptionally (PTGS). Duc
 kweeds unique reduced morphology and reproduction\, and a wide gradient of
  genome size and TE content from the TE-poor Spirodela polyrhiza (~138 Mb\
 , ~20% TEs) to the TE-rich Wolffia (~850 Mb\, ~75% TEs)\, makes them a pow
 erful comparative system to ask how a simplified body plan\, a clonal life
 style and contrasting genome organization shape plant RNA and epigenetic s
 ilencing.\n\nSeveral studies have shown that duckweeds run a streamlined s
 ilencing machinery with uneven losses. The RdDM and transcriptional-silenc
 ing arm is simplified while the antiviral/PTGS arm lacks as well several k
 ey silencing factors. In Spirodela and Wolffia\, this reduced toolkit stil
 l resolves TEs sharply: silencing and DNA methylation concentrate on young
 \, intact elements\, and the contrasting TE loads of the two species reorg
 anize their small RNA and methylation landscapes accordingly. Across these
  analyses siRNAs of all size classes are frequently produced from the same
  loci\, indicating that the pathways might be wired differently from the c
 anonical pathways stablished in Arabidopsis rather than simply reduced.\n\
 nhttps://indico.unina.it/event/117/contributions/2973/
LOCATION:Department of Agricultural Sciences of the University of Napoli F
 ederico II\, Portici\, Italy Sala Cinese
URL:https://indico.unina.it/event/117/contributions/2973/
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