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SUMMARY:Constructing designer duckweed holobionts for comprehensive analys
 es of plant-microbe interactions
DTSTART;VALUE=DATE-TIME:20260930T064500Z
DTEND;VALUE=DATE-TIME:20260930T071500Z
DTSTAMP;VALUE=DATE-TIME:20260915T194341Z
UID:indico-contribution-434-2889@cern.ch
DESCRIPTION:Speakers: Eric Lam (Department of Plant Biology\, Rutgers the 
 State University of New Jersey\, New Brunswick)\nHolobiont refers to a hol
 istic view of the organism as a combination of an eukaryote and its associ
 ated microbiome\, which is a dynamic assortment of microbes inhabiting var
 ious niches that the host presents. The bacterial microbiome of water lent
 ils (aka duckweeds) has been characterized over the past decade and may en
 hance or inhibit the growth of these aquatic plants. Most of these studies
  involved presenting isolates of duckweed-associated bacteria (DABs) to gn
 otobiotic duckweeds and observing their effects on the plant’s behavior 
 after days or weeks. However\, the mechanism of how DABs can modulate duck
 weed growth in different context is still lacking due to the complexity of
  the interacting partners as well as with the changing environmental conte
 xt during the assays. Leveraging the clonal propagation lifestyle of duckw
 eeds\, we have constructed duckweed holobionts (DHBs) using characterized 
 DABs and their combinations that stably colonize gnotobiotic Lemna minor 5
 576\, a duckweed strain with a well characterized genome. These associatio
 ns may be stably propagated for months or years under laboratory condition
 s\, showing that assembled DABs could be transferred between generations o
 f fronds. Preliminary studies show that these designer DHBs can provide a 
 convenient platform to study how stable colonization by individual DABs an
 d their mini-communities can modify the host’s physiological properties\
 , such as their relative growth rate and levels of vitamin B12\, as well a
 s the response of plant-microbe association to environmental factors. The 
 DHB approach can thus help generate deep datasets on plant-microbe interac
 tions while producing value-added strains as candidates for practical appl
 ications.\n\nhttps://indico.unina.it/event/117/contributions/2889/
LOCATION:Department of Agricultural Sciences of the University of Napoli F
 ederico II\, Portici\, Italy Sala Cinese
URL:https://indico.unina.it/event/117/contributions/2889/
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