BEGIN:VCALENDAR VERSION:2.0 PRODID:-//CERN//INDICO//EN BEGIN:VEVENT SUMMARY:UHPLC-MS/MS profiling and biological activities of phenolic compou nds from three duckweed species for the identification of potential health -promoting biomolecules DTSTART;VALUE=DATE-TIME:20261001T100000Z DTEND;VALUE=DATE-TIME:20261001T101500Z DTSTAMP;VALUE=DATE-TIME:20260916T013402Z UID:indico-contribution-441-2926@cern.ch DESCRIPTION:Speakers: Anim Ujong (Technological University Dublin and Teag asc Food Research Centre)\nDuckweeds have gained increasing attention as s ustainable sources of bioactive compounds with promising applications in f unctional foods and nutraceuticals. This study comprehensively characteriz ed the phenolic profiles of three duckweed species (Lemna minor\, Lemna gi bba\, and Spirodela polyrhiza) using ultra-high-performance liquid chromat ography coupled with tandem mass spectrometry (UHPLC-MS/MS). The bioactive potential of the extracts was evaluated through in vitro antioxidant\, en zyme inhibitory\, anti-inflammatory and ACE-inhibitory assays to assess th eir functional properties. Targeted UHPLC-MS/MS analysis revealed distinct species-specific phenolic profiles\, with quinic acid identified as the p redominant compound in all species\, particularly in L. minor (3.99 µg/mg extract DM)\, followed by L. gibba (2.31 µg/mg extract DM) and S. polyrh iza (1.64 µg/mg extract DM). S. polyrhiza exhibited a characteristic flav onoid-rich profile\, with markedly higher concentrations of luteolin-7-O-g lucoside (2.11 µg/mg extract DM) and vitexin (2.13 µg/mg extract DM) com pared with L. minor (0.47 and 0.20 µg/mg extract DM\, respectively) and L . gibba (0.12 and 0.10 µg/mg extract DM\, respectively). Apigenin was det ected exclusively in S. polyrhiza\, whereas caffeic acid was most abundant in L. gibba (0.49 µg/mg extract DM). Among the three species\, L. minor demonstrated the highest total phenolic content (23.54 mg GAE/g DW)\, ferr ic reducing antioxidant power (FRAP\; 43.55 mmol Fe²⁺/g DW). Principal component analysis revealed strong associations between specific phenolic constituents and measured biological activities\, suggesting that species- specific phenolic profiles contribute to the functional properties of duck weed extracts. These findings highlight duckweed as a promising sustainabl e source of phenolic compounds with potential applications as functional f ood ingredients and health-promoting nutraceuticals.\n\nhttps://indico.uni na.it/event/117/contributions/2926/ LOCATION:Department of Agricultural Sciences of the University of Napoli F ederico II\, Portici\, Italy Sala Cinese URL:https://indico.unina.it/event/117/contributions/2926/ END:VEVENT BEGIN:VEVENT SUMMARY:Synergistic Nutrient Formulations and Thermal Drying Strategies fo r Enhanced Duckweed Nutrition DTSTART;VALUE=DATE-TIME:20261001T101500Z DTEND;VALUE=DATE-TIME:20261001T103000Z DTSTAMP;VALUE=DATE-TIME:20260916T013402Z UID:indico-contribution-441-2925@cern.ch DESCRIPTION:Speakers: Derek Juinn Chieh Chan (Universiti Sains Malaysia)\n Duckweeds are gaining attention as sustainable nutrient-rich biomass due t o their exceptional capacity to accumulate proteins and carbohydrates. How ever\, maximizing biomass production alone does not guarantee the preserva tion of nutritional quality after harvest. This study investigated both cu ltivation and post-harvest processing conditions for three duckweed specie s (*Lemna minor*\, *Spirodela polyrhiza*\, and *Wolffia globosa*) using a two-phase approach. During cultivation\, single and sequential nutrient me dia with different nitrogen-to-phosphorus (N:P) ratios were evaluated over 12–28 days. Distinct species-specific responses were observed. *L. mino r* achieved the highest relative growth rate (0.138 day⁻¹) and protein content (50.2% dry weight) under a sequential shift from a high to low N:P ratio (18:1 to 6:1). *S. polyrhiza* produced the highest protein concentr ation overall (51.7% dry weight) and performed best under prolonged cultiv ation at a low N:P ratio (6:1). In contrast\, *W. globosa* exhibited slowe r growth (0.071 day⁻¹) but accumulated the greatest lipid content (6.2% ) and carbohydrate content (52.4% dry weight) during extended cultivation at a high N:P ratio (18:1). Drying temperature significantly affected bioc hemical composition. Lower temperatures (40–50°C) better preserved prot ein integrity\, maintaining protein yields of approximately 300–340 mg g ⁻¹ while minimizing lipid oxidation. Higher temperatures (50–60°C) p romoted degradation of non-carbohydrate components\, increasing apparent c arbohydrate concentrations (>400 mg g⁻¹) and enhancing anthocyanin extr actability from approximately 12 to over 43 mg g⁻¹.These results demons trate that species-specific nutrient management combined with low-temperat ure drying (40–50°C) is essential for preserving duckweed biomass quali ty and supporting large-scale\, sustainable production systems\n\nhttps:// indico.unina.it/event/117/contributions/2925/ LOCATION:Department of Agricultural Sciences of the University of Napoli F ederico II\, Portici\, Italy Sala Cinese URL:https://indico.unina.it/event/117/contributions/2925/ END:VEVENT BEGIN:VEVENT SUMMARY:Comparison of Ultrasound- and Pulsed Electric Field-Assisted Prote in Extraction from fresh Lemna minor: Process Evaluation and Functional Ch aracterization DTSTART;VALUE=DATE-TIME:20261001T094500Z DTEND;VALUE=DATE-TIME:20261001T100000Z DTSTAMP;VALUE=DATE-TIME:20260916T013402Z UID:indico-contribution-441-2924@cern.ch DESCRIPTION:Speakers: Patricia Maag (Hochschule Weihenstephan-Triesdorf Un iversity\, TU Berlin)\nEfficient extraction methods are required to maximi ze protein recovery from duckweed (L. minor) while preserving nutritional quality and functionality. The Institute of Food Technology at Weihensteph an-Triesdorf University of Applied Sciences compared ultrasound-assisted e xtraction (UAE) and pulsed electric field-assisted extraction (PEF) with c onventional alkaline–acidic extraction.\nFresh greenhouse-grown L. minor was processed immediately after harvest. UAE parameters were optimized\, while PEF conditions were adopted from previous work. Protein concentrates were characterized for protein content and yield\, chlorophyll\, antioxid ant capacity\, and techno-functional properties.\nUAE achieved the highest protein extraction efficiency at 100% amplitude and 2 min sonication\, wh ereas the investigated PEF treatment (5 min homogenization prior to 5kV/cm ) comparatively enhanced cell permeabilization. UAE and PEF yielded protei n concentrates containing 60.81% and 65.24% protein\, with protein yields of 55.02% and 34.70%\, respectively. Conventional extraction produced the lowest protein yield (30.78%) but the highest protein content (66.19%)\, w hich did not differ significantly from PEF. Extraction technology also inf luenced pigment recovery\, antioxidant capacity\, and techno-functional pr operties. UAE achieved the highest chlorophyll extraction by ~64% and pref erentially recovered hydrophilic antioxidants\, consistent with previous f indings [1]\, whereas PEF favored hydrophobic antioxidants. The PEF-derive d protein concentrate showed the highest protein solubility and superior f oaming and emulsifying capacities\, while UAE improved foam and emulsion s tability.\nThese findings demonstrate that UAE and PEF are promising alter natives to conventional extraction\, enabling the production of duckweed p rotein concentrates with tailored compositional and techno-functional prop erties for diverse food applications.\n\nhttps://indico.unina.it/event/117 /contributions/2924/ LOCATION:Department of Agricultural Sciences of the University of Napoli F ederico II\, Portici\, Italy Sala Cinese URL:https://indico.unina.it/event/117/contributions/2924/ END:VEVENT BEGIN:VEVENT SUMMARY:Fog-o-ponics\; a novel duckweed cultivation method DTSTART;VALUE=DATE-TIME:20261001T093000Z DTEND;VALUE=DATE-TIME:20261001T094500Z DTSTAMP;VALUE=DATE-TIME:20260916T013402Z UID:indico-contribution-441-2890@cern.ch DESCRIPTION:Speakers: Marcel A.K. Jansen (1School of Biological\, Earth an d Environmental Sciences\, University College Cork\, Cork\, Ireland 2Susta inable Institute\, University College Cork\, Cork\, Ireland)\nInnovative c ultivation techniques are required for new circular economy applications t hat require cultivation of large surface areas of duckweed under indoor co nditions. To increase duckweed cultivation area relative to floor space\, multilayered cultivation systems have been developed. In these stacked sys tems\, duckweed is cultivated on liquid medium\, an approach inspired by t he natural\, floating habit of these aquatic plants. LEDs are sandwiched b etween each cultivation layer to provide homogenous light conditions. Stac ked systems can successfully generate large amounts of duckweed biomass\, but their construction involves an engineering challenge due to the sheer weight of the water column and associated mechanical support structures. A s a result\, most stacked systems comprise less than 10 cultivation layers . As an alternative\, growth of duckweed in a mist of nutrient-supplemente d fine droplets\, i.e. fog-o-ponics\, was pioneered. The advantage of fog- o-ponic cultivation of duckweed is that trays with water are omitted and r eplaced by a thin layer of fabric on which the duckweed is positioned. Fog droplets pass through the hydrophobic fabric\, to be returned to the mist er\, creating a circular flow-thru system. The use of fabrics substantiall y decreases the weight and thickness of each layer of duckweed in a stacke d fog-o-ponic cultivation system\, and as a consequence enables increases in the number of duckweed layers per unit of floor space. Lemna minor was grown in a fog-o-ponic system comprised of 10 cultivation layers in an inc ubator of just 40 cm height\, demonstrating good growth and an attractive metabolic profile\, as well as upscaling potential.\n\nhttps://indico.unin a.it/event/117/contributions/2890/ LOCATION:Department of Agricultural Sciences of the University of Napoli F ederico II\, Portici\, Italy Sala Cinese URL:https://indico.unina.it/event/117/contributions/2890/ END:VEVENT END:VCALENDAR