Speaker
Francesca Gorini
Description
8-Oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG), a key product of DNA oxidation, has been identified as an epigenetic factor that influences the transcription process. However, the precise role of 8-oxodG in transcription regulation is not fully established.
To investigate the involvement of 8-oxodG in global transcription regulation, we applied two complimentary strategies. First, we inhibited RNAPII activity to determine if the presence of 8-oxodG is dependent on RNAPII mobility. Second, we reduced the amount of genomic 8-oxodG to see how it affected RNAPII mobility.
Our findings indicate that 8-oxodG actively enhances transcription rather than being a passive participant.
| Department | Dept Molecular Medicine and Medicine Biotechnology |
|---|
Primary authors
Francesca Gorini
Giovanni Scala
(University Federico II)
SUSANNA AMBROSIO
(Federico II)
Prof.
Luigi Lania
(University Federico II of Naples)
Prof.
Barbara Majello
(Dipartimento di Biologia - Università degli studi di Napoli Federico II)
Stefano Amente
(University Federico II)
