Speaker
Description
Potential cobalamin (vitamin B12)-producing Wolffia globosa-associated bacteria were investigated through shotgun metagenomic sequencing analysis. Taxonomic classification assigned microbiomes consisting of 5 phyla, 8 classes, 36 families, and 68 genera. High-quality metagenome-assembled genomes (MAGs) were assigned to Allorhizobium sp., Alsobacter sp., Bosea sp., Bradyrhizobium huaxiensis, Brevundimonas sp., Methylophilus sp., Microbacterium sp., Mycobacterium sp., Neoroseomonas marina, Nevskia sp., Novosphingobium sp., Phenylobacterium sp., Pseudonocardia carboxydivorans, Rhizobium sp., Rhizorhabdus sp., Roseomonas sp., Sphingomonas sp., and Xanthomonas sontii. Functional annotation across all MAGs revealed potential pathways related to aromatic compound degradation, cofactor and vitamin metabolism, serine and threonine metabolism, nitrogen metabolism, and other carbohydrate metabolic processes. Several MAGs contained complete modules of cobalamin biosynthesis including hem, cob, cbi genes responsible for corrin ring synthesis, cobalt transport, lower-ligand formation, and final cobalamin assembly. Interestingly, the definitive molecular marker for active B12 production, bluB, was identified in several MAGs. Subsequently, eight bacterial genera were isolated using culture dependent methods and three novel species have been proposed. Co-cultivation experiments are underway to understand the associated B12 production of W. globosa holobionts.
Keywords
W.globosa; microbiome; MAGs; cobalamin; novel_species
References
Manuscript in preparation.
| Corresponding author email | arinthip.t@ku.ac.th |
|---|---|
| Scientific Session | Microbiome & Interactions |
