Speaker
Description
Vaccination is a cornerstone of modern health management. It`s particular important in intensive animal production systems, such as aquaculture, where high stocking densities facilitate the rapid spread of pathogens. Oral vaccines offer a stress-free, scalable alternative to traditional fish injections. Our study explores duckweed as platform for vaccine production and as vehicle for oral administration. Using a deconstructed potato virus X-based transient expression system [1], recombinant proteins analogs of antigens of koi herpesvirus (KHV) and carp edema virus (CEV) were expressed in duckweed. Freeze-drying was shown to preserve the recombinant antigens in the duckweed biomass, enabling long-term storage at room temperature. Obtained freeze-drying duckweed biomass expressing KHV antigens was used for carp immunization via intraperitoneal injection and intubation [2]. Both methods of administration induced release of the specific KHV-neutralising antibodies. Such data provide a strong support for the efficacy of duckweed as producer of oral vaccines. Transient expression of recombinant proteins in duckweed can facilitate and accelerate the development of oral vaccines for animals.
Keywords
transient transgene expression, oral vaccines
References
[1] Peterson, A., Kishchenko, O., Zhou, Y., Vasylenko, M., Giritch, A., Sun, J., et al. (2021). Robust Agrobacterium-mediated transient expression in two duckweed species (Lemnaceae) directed by non-replicating, replicating, and cell-to-cell spreading vectors. Front. Bioeng. Biotechnol. 9, 761073. doi: 10.3389/fbioe.2021.761073
[2] Kishchenko, O., Peterson, A., Adamek, M., Schroers, V. J., Piačková, V., Korytář, T., et al. (2026). Fish immunization by duckweed biomass accumulating recombinant cyprinid herpesvirus 3 antigens induces specific immune response. New Biotechnol. 94, 272–282. doi: 10.1016/j.nbt.2026.06.011
| Corresponding author email | kishchenko@ipk-gatersleben.de |
|---|---|
| Scientific Session | Applications |
